Dnasip

DNA stable isotope probing (DNA-SIP) methods are more user-friendly because of sequence information being so widely available, as well as the ease of nucleic acid extraction from environmental samples. Stable isotope-labeled DNA can be isolated from mixed microbial communities on the basis of the increase in buoyant density associated with isotopic enrichment. Density centrifugation in cesium chloride gradients was used to separate heavy DNA from natural DNA, and 16S rDNA clone libraries constructed from heavy DNA were sequenced to obtain the identity of organisms assimilating the 13C-labeled substrate used in the study. This technique has been used to attribute particular members of the Proteobacteria with methanol utilization in an oak forest soil (88).

Organic Gardeners Composting

Organic Gardeners Composting

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