For the detection of dioxin and related halogenated aromatic hydrocarbons

Michael S. Denison, Scott R. Nagy, Michael Ziccardi, George C. Clark, Michael Chu, David J. Brown, Guomin Shan, Yukio Sugawara, Shirley J. Gee, James Sanborn, and Bruce D. Hammock

Abstract Proper epidemiological, risk assessment and exposure analysis of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD, dioxin) and related halogenated aromatic hydrocarbons (HAHs) requires accurate measurements of these chemicals in the species of interest and in various exposure matrices, i.e., biological, environmental, and food. High-resolution instrumental analysis techniques are established for these chemicals; however, these procedures are costly and time consuming, and as such, they are impractical for large-scale sampling studies, i.e., for epidemiological studies and assessment of areas with widespread contamination. Accordingly, we have developed two

Contents

I. Introduction

485 488 490

II. Tier 1: CALUX bioassay

III. Tier 2: TCDD immunoassay

IV. Application of CALUX and TCDD immunoassay. Acknowledgments

References

simple, rapid, sensitive, and inexpensive assays (a TCDD immunoassay and a novel recombinant cell bioassay called CALUX) for the detection and relative quantitation of TCDD and related HAHs in extracts of a variety of matrices. These assays can be used individually or in combination as relatively rapid prescreening tools to identify positive samples for subsequent instrumental analysis and to drive chemical fractionation of complex mixtures in order to identify novel chemicals or classes of chemicals which exert dioxin-like activity. The availability of these bioanalytical approaches should greatly facilitate large-scale sampling studies needed for the accurate assessment of exposure to this ubiquitous class of environmental contaminants.

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