Comparison of SHS and HSSE in the analysis of fragrances

The selection of an HS technique strongly depends on the sensitivity required which is linked to the instrumentation, and especially to the detectors available in the laboratory. In our experience [22], of the recently introduced HS techniques, ITEX, SPME and HSSE, the latter is the most sensitive technique and enrichment factors compared to SHS are increased drastically. This is illustrated with the analysis of some fragrances in a lotion. In Figure 4A, the total ion chromatogram using classical SHS sampling is shown. The internal standards, added at the same concentration level, are detected at 10.3 and 23.1 min, respectively. The response for the first internal standard is higher in comparison to the second internal standard, corresponding to their relative volatility. In this chromatogram, linalool (peak 1) and hexyl cinnamaldehyde (peak 6) are easily detected. Other solutes are only detected as traces and confirmation of their presence by mass spectral comparison with a library spectrum is difficult. The chromatogram obtained by HSSE sampling on PDMS is shown in Figure 4B. Excellent enrichment is obtained considering that the abundances in both chromatograms differ by a factor 10. The structures of all solutes could be elucidated through the MS spectra and some important compounds are citronellol (peak 2), alpha isomethyl ionone (peak 3), lilial (peak 4) and amyl cinnamaldehyde (peak 5).

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