Surface Plasmon Resonance Immunosensor

A surface plasmon resonance (SPR) immunoassay for the detection of TNT was developed by using an immunoreaction between 2,4,6-trinitrophenol-ovalbumin (TNP-OVA) conjugate and anti-TNP antibody (Shankaran et al. 2005).

The quantification of TNT is based on the principle of indirect competitive immunoassay, in which the immunoreaction between the TNP-OVA conjugate and anti-TNP antibody was inhibited of free TNT in solution. The decrease in the resonance angle shift is proportional to an increase of TNT concentration and the immunoassay exhibited excellent sensitivity for the detection of TNT. The SPR sensor showed a very low sensitivity to some structurally related nitroaromatic derivatives, such as 24DNT, 2ADNT, 4ADNT, and 1,3-dinitrobenzene. A modified TNT sensor has been further fabricated for highly sensitive SPR detection of TNT (Mizuta et al. 2008).

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